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: Confirming Soybean Rust Identification of soybean rust will be critical for proper management actions to be taken. You are encouraged to contact your local University of Nebraska Cooperative Extension office if you are uncertain about a symptom in your field. For confirmation of an unkown sample in Nebraska please submit a leaf sample containing several leaves to the Plant & Pest Diagnostic Clinic. Any abuse of this system with sample submission for symptoms which are not likely to be confused with soybean rust will be billed. Scouting Sample check list for soybean rust: * Use a sealed plastic bag for leaf samples. Tips to identification: Using a hand lens is critical to identify soybean rust. The first time will be much more difficult until you gain experience.
Lesions of soybean rust under a 16X handlens.
Suspected leaves can be placed in a sealed plastic bag with moist toweling for a make-shift humidity chamber. The optimum temperature is from 65 to 80 F, so room temperature will typically work well. Within 24 to 72 hours (depending on lesion maturity) sporulation should be evident on the lesion if the leaf has not been treated with a fungicide or the residual activity has lapsed. Rust I.D. in Brazil. Federal and Non-Federal Responsibilities for identification: The current responsibilities of parties identifying soybean rust can be found in the document ”Federal/Nonfederal Responsibilities for Identification of Phakopsora pachyrhizi”
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